Biotechnology

Topic wise MCQ test for MHT-CET and NEET

12. Biotechnology         

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(1) The bacterium which causes a plant disease called crown gall is ……… .

(a) Helicobacter pylori            

(b) Agrobacterium tumifaciens

(c) Thermophilus aquaticus     

(d) Bacillus thuringienesis

Answer:-  (b) Agrobacterium tumifaciens

(2) The enzyme nuclease hydrolyses ……… of polynucleotide chain of DNA.

          (a) hydrogen bond

(b) phosphodiester bonds

(c) glycosidic bonds                

(d) peptide bonds

Answer:-  (b) phosphodiester bonds

 

(3) In vitro amplification of DNA or RNA segment is known as ……… .

(a) chromatography                          

(b) southern blotting      

(c) polymerase chain reaction            

(d) gel electrophoresis

Answer:- (c) polymerase chain reaction

 

(4) Which of the following is the correct recognition sequence of restriction enzyme hind III.

(a)     5’ ---A-A-G-C-T-T---3’

3’ ---T-T-C-G-A-A---5’

(b)     5' ---G-A-A-T-T-C---3'

3' ---C-T-T-A-A-G---5'

(c)      5' ---C-G-A-T-T-C---3'

3' ---G-C-T-A-A-G---5'

(d)     5' ---G-G-C-C---3'

3' ---C-C-G-G---5'

Answer:-  (a)        5’ ---A-A-G-C-T-T---3’

3’ ---T-T-C-G-A-A---5’

 

(5) Recombinant protein ......... is used to dissolve blood clots present in the body.

(a) insulin   

(b) tissue plasminogen activator         

(c) relaxin  

(d) erythropoietin

Answer:-  (b) tissue plasminogen activator 

 

(6) Recognition sequence of restriction enzymes are generally ............... nucleotide long.

(a) 2 to 4    

(b) 4 to 8    

(c) 8 to 10  

(d) 14 to 18

Answer:-   (b) 4 to 8

 

(7) Recombinant DNA technique was established by ……… .

(a) Herbert Boyer

(b) Stanley Cohen          

(c) Peter Lobban  

(d) both (a) and (b)

Answer:- (d) both (a) and (b)

 

(8) Modern biotechnology includes ……… .

(a) r-DNA technology and PCR         

(b) microarrays, cell culture and fusion

(c) production of curds, cheese, wine           

(d) both (a) and (b)

Answer:- (d) both (a) and (b)

 

(9) How many of the following statements are correct with reference to electrophoresis.

1. DNA is positively charged and it moves towards the negative electrode.

2. DNA is negatively charged and it moves towards the anode.

3. Longer DNA fragments move faster during electrophoresis.

4. Shorter DNA fragments move slowly during electrophoresis.

(a) One       

(b) Two      

(c) Three    

(d) Four

Answer:-  (a) One

 

(10) PCR technique was developed by ……… .

(a) Stanley Cohen

(b) Herbert Boyer

(c) W. Arber         

(d) K. Mullis

Answer:-  (d) K. Mullis

 

(11) ……… technique is used for in vitro gene cloning or gene multiplication.

(a) Electrophoresis         

(b) SDS-PAGE    

(c) Spectroscopy  

(d) PCR

Answer:-   (d) PCR

 

(12) During denaturation step of polymerase chain reaction, the reaction mixture is heated at

(a) 40 – 60°C       

(b) 70 – 75°C       

(c) 90 – 98°C       

(d) 36°C

Answer:- (c) 90 – 98°C 

 

(13) Annealing temperature of primer is ……… .

(a) 40 – 60°C       

(b) 72°C     

(c) 90 – 98°C       

(d) 36°C

Answer:-  (a) 40 – 60°C

 

(14) During PCR new strand of DNA is synthesized by ……… .

(a) thermostable Taq DNA polymerase       

(b) ligase    

(c) phosphorylase 

(d) RE

Answer:-  (a) thermostable Taq DNA polymerase

 

(15) Restriction enzymes were discovered by ……… .

(a) K. Mullis        

(b) S. Cohen         

(c) H. Boyer         

(d) Smith, Nathan and Arber

Answer:-  (d) Smith, Nathan and Arber

 

(16) The molecular scissors of DNA are ……… .

(a) ligases   

(b) polymerases   

(c) endonucleases          

(d) transcriptases

Answer:-  (c) endonucleases   

 

(17) ……… generates DNA fragments with sticky ends.

(a) Eco RI            

(b) Bam HI           

(c) Hind II            

(d) both (a) and (b)

Answer:- (d) both (a) and (b)

 

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(18) ……… is Type III endonulcease.

(a) HapaI             

(b) Eco R I           

(c) BgII                

(d) Eco K

Answer:-  (a) HapaI      

 

(19) Select the incorrect statement.

(a) Type II restriction endonucleases have separate activities for cleaving and methylation.

(b) Type I restriction endonucleases function simultaneously as endonuclease and methylase.

(c) Type II restriction endonucleases cut DNA at specific non–pallindromic sequences.

(d) Thousands of Type II restriction endonucleases have been discovered.

Answer:-  (c) Type II restriction endonucleases cut DNA at specific non–pallindromic sequences.

 

(20) Ti plasmids are present in ……… .

(a) Bacillus thuringiensis                  

(b) Agrobacterium tumefaciens

(c) Haemophilus influenza                

(d) Escherichia coli

Answer:-  (b) Agrobacterium tumefaciens

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